General Cell Lines and Cell Cultures
Lonza Walkersville EGM Endothelial Cell Growth Medium BulletKit
Choose from a variety of endothelial cell growth media for an easy cultivation of primary endothelial cells. Every endothelial cell growth medium has its special formulation and is optimized to grow certain cell types. Each type of endothelial cell growth medium is guaranteed to perform when used to grow Clonetics Primary Cells. Content:1 x EBM Basal Medium (CC-3121), 500 mL1 x EGM SingleQuots Supplement Pack (CC-4133) containing:1 x Green Cap Vial with hEGF 0.50 mL1 x Natural Cap Vial with Hydrocortisone 0.50 mL1 x Red Cap Vial with GA-1000 0.50 mL1 x Bottle BBE 2.00 mL1 x Blue Cap Vial with Ascorbic Acid 0.50 mL1 x Bottle FBS 10.00 mL
Cellular Dynamics International iCELL Hepatocytes 2.0 Kit, Cryopreserved single-cell suspension, ≥10.0 x 10^6 viable cells per kit, 1.5 ml cryovials, >95% purity
iCELL Hepatocytes 2.0 Kit, Cryopreserved single-cell suspension, ≥10.0 x 10^6 viable cells per kit, 1.5 ml cryovials, >95% purity
Lonza Walkersville HUVEC Single Donor in EGM-Plus
HUVECs cultured in EGM-Plus Growth Media offer faster proliferation rates while maintaining the same high quality characterization as HUVECs in EGM Media Lonza s Cryopreserved HUVECs are shipped as frozen primaries They are guaranteed to express CD31/105 and survive in culture through 15 population doublings ( except HUVEC-XL guaranteed through 5 PD) All cells test negative for mycoplasma bacteria yeast and fungi HIV-1 hepatitis B and hepatitis C are not detected for all donors and/or cell lots A Certificate of Analysis is provided for each cell lot purchased Our pooled vs single donor options allow researchers to study or exclude donor variability from their experiments Depending upon the study pooled version is typically utilized to reduce donor variability while single donor option allows researchers to assess donor-specific differences HUVEC-XL size consists of 10 million cells/amp achieved by pooling donors
iQ Biosciences Human Natural T Regulatory Cells, 1M cells/vial
High-quality, high-viability (typically > 80%) natural human T regulatory cells isolated from PBMCs with confirmed suppressive capacity. Used for a wide variety of in-vitro applications, including co-culture suppression and cytotoxicity assays. All orders come with an iQ Certificate of Analysis.
Sciencell Research Laboratories Human Astrocytes 1 x 10^6 cells in 1 ml volume.
Isolated from human brain (cerebral cortex). HA are cryopreserved at passage one and delivered frozen. Each vial contains >1 x 10^6 cells in 1 ml volume. Storage: Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture is needed for experiments.
Lonza Walkersville SG Cell Line 96-well Nucleofector Kit (960 RCT)
The SG Cell Line 96-well Nucleofector Kit is one of our three kits suited for medium-throughput transfection of cell lines when working with the 4D-Nucleofector 96-well Unit.Benefits:High transfection efficiencies in difficult-to-transfect cell linesCompatible with liquid handling systems for automated high-throughput electroporationConditions are transferable between 4D-Nucleofector System, 4D-Nucleofector 96-well Unit and 384-well Nucleofector SystemApplications:Transfection of low cell numbers in the range of 5 x 10⁴ to 1 x 10⁶ cellsHigh-throughput applications, e.g. screens of arrayed cDNA, RNAi or CRISPR librariesContent:22.5 mL SG Cell Line Nucleofector Solution5 mL Supplement 150 µg pmaxGFP Vector (1 μg/μl in 10 mM Tris pH 8.0)10 x 96-well Nucleocuvette Plate (20 μl)
Eurofins Discoverx PathHunter U2OS SREBP2 Nuclear Translocation Cell Line, 2 vials. (93-0830C3)
The PathHunter U2OS SREBP2 Nuclear Translocation Cell Line is a stable, engineered cell line for use in studying drug candidates affecting the cholesterol biosynthesis pathway. This cell line enables assessment of ligand (e.g. 5'-UMP) based pathway activation via detection of resulting SREBP2 nuclear translocation. This product uses EFC technology. The included cell line overexpresses ProLabel-tagged SREBP2 and nuclear localized EA. Upon pathway activation, induced nuclear translocation of SREBP2 leads to production of EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/pathways.
Eurofins Discoverx PathHunter CHO-K1 MTNR1A β-Arrestin Cell Line, 2 vials. (93-0951C2)
The PathHunter MTNR1A cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. 2-iodomelatonin) based activation of MTNR1A GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged MTNR1A and EA-tagged β-Arrestin-2. Activation of MTNR1A stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.
Lonza Walkersville Hu Coro Artery Endo Nufe Kit
The Nucleofector 2b System (or older generations I or II) works cell type specific kits each of them dedicated to an individual primary cell This Nucleofector Kit is the optimal kit for efficient transfection of primary human coronary artery endothelial cells (HCAECs) The respective cell type-specific Optimized Protocol or recommendation is available under Instructions or in our Lonza Bioscience knowledge database In this Optimized Protocol the best Nucleofection conditions are indicated In addition we share our experience and knowledge for treatment of this cell type
Eurofins Discoverx PathHunter U2OS ErbB2/ErbB4 Dimerization Cell Line, 2 vials. (93-0960C3)
The PathHunter ErbB2/ErbB4 Dimerization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This cell line enables assessment of ligand (e.g. heregulin β-1) induced dimerization of ErbB2/ErbB4 receptors. This product uses EFC technology. The cell line included overexpresses ErbB2 target protein fused to PK alongside ErbB4 target protein fused to EA. Upon ligand-induced dimerization of ErbB2/ErbB4, detectable EFC signal is produced. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/kinases.
Eurofins Discoverx PathHunter DLD1 FFAR1 β-Arrestin Cell Line, 2 vials. (93-0950C13)
The PathHunter FFAR1 cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. free fatty acids) based activation of FFAR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged FFAR1 and EA-tagged β-Arrestin-2. Activation of FFAR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. This stable modified cell line is provided as two vials of cryopreserved cells. For detailed product information, User Manual and datasheet, visit www.discoverx.com/gpcr.